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Signal Peptide Prediction
Signal peptides target proteins to the extracellular environment either through direct plasmamembrane translocation in prokaryotes or are routed through the Endoplasmatic Reticulum in eukaryotic cells. The signal peptide is removed from the resulting mature protein during translocation across the membrane.
For prediction of signal peptides, we query SignalP [Nielsen et al., 1997], [Bendtsen et al., 2004a] located at http://www.cbs.dtu.dk/services/SignalP/. Thus an active internet connection is required to run the signal peptide prediction.
These organisms can be selected:
- Eukaryote
- Gram-negative bacteria
- Gram-positive bacteria
The predictions obtained can either be shown as annotations on the sequence or be shown as the detailed and full text output from the SignalP method. This can be used to interpret borderline predictions.
You can perform the analysis on several protein sequences at a time. This will add annotations to all the sequences and open a view for each sequence if a signal peptide is found
After running the prediction as described above, the protein sequence will show predicted signal peptide as annotations on the original sequence.
Each annotation will carry a tooltip note saying that the corresponding annotation is predicted with SignalP version 3.0. Additional notes can be added, and undesired annotations can be removed through the edit annotation feature
Additional information on SignalP and Center for Biological Sequence analysis (CBS) can be found at http://www.cbs.dtu.dk and in the original research paper [Nielsen et al., 1997], [Bendtsen et al., 2004a].
