Protein Charge

You can create a graph of one the electric charge as a function of pH for one or more sequences at a time. This is particularly useful for finding the net charge of the protein at a given pH. This knowledge can be used e.g. in relation to isoelectric focusing on the first dimension of 2D-gel electrophoresis. The isoelectric point (pI) is found where the net charge of the protein is zero.

The calculation of the protein charge does not include knowledge about any potential post-translational modifications, the protein may have.

You can perform the analysis on several protein sequences at a time. This will result in one plot with a graph for each protein selected.

These preferences can be applied to the whole graph:

  • Frame around the graph (yes/no)
  • Location of the X-axis and Y-axis
  • Location of the ticks (outside or inside the axes)
  • Show grid behind the graph? (none/major ticks/all ticks)

These preferences can be applied individually to each graph:

  • Dot type (none/cross/square/diamond/circle/dot/etc.)
  • Dot color
  • Line width (thin/medium/wide)
  • Line type (none/line/long dash/short dash)
  • Line color

The protein charge plot may be saved, printed or exported to PDF or a graphics format.

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